Mast cells are immune cells that essentially contribute to various inflammatory processes. Ca2+ entry and cytosolic Ca2+ rise is essential for inflammatory mediator release from mast cells. Adenine nucleotides (AN) such as adenosine, ATP or NAADP regulate mast cell Ca2+ homeostasis and function via endolysosomal two-pore channels (TPCs) and plasmalemmal channels. We will apply (endolysosomal) patch-clamp electrophysiology, imaging techniques, cell biological assays and mouse models to investigate the role of Orai and TPC channels and novel interaction partners of these for mast cell-mediated inflammation.
Subproject of: SFB 1328: Adenine Nucleotides in Immunity and Inflammation
Project Heads: Professor Dr. Marc Freichel; Professor Dr. Christian Michael Grimm